Journal: Journal of Molecular Cell Biology

Article Title: Ablation of Zfhx4 results in early postnatal lethality by disrupting the respiratory center in mice

doi: 10.1093/jmcb/mjaa081

Figure Lengend Snippet: Math1 is coexpressed with Zfhx4 and its expression levels are decreased in Zfhx4 -null mouse hindbrains. ( A ‒ H ) In P0 Math-Cre mice, neurons expressing Math1 were monitored by immunofluorescent staining of Cre. Codistribution of Zfhx4 with Cre in the cerebellum (Cb, A ‒ D ) and paratrigeminal region ( E ‒ H ) of P0 Math-Cre mice. DAPI was used to stain nuclei. Insets are magnified neurons showing Zfhx4 (green), Cre (red), and coexpression of Zfhx4 with Math1 (selected regions indicated by solid white rectangles). ( I ‒ J ”) Immunofluorescent staining and quantification for Math1 (green) in the Cb and pon (Pn) from P0 WT and Zfhx4 -null mice. Insets are magnified views of the selected regions indicated by solid white rectangles. Math1 expression decreased in both Cb and Pn of P0 Zfhx4 -null mice. Scale bar, 100 μm and 25 μm (insets). Data in bar graphs are expressed as mean ± SD. **** P < 0.0001 (Student’s t -test), n = 15.

Article Snippet: The antibodies and dilutions used in this study were as follows: Zfhx4 (1:2000), Phox2b (1:150, Santa Cruz), Math1 (1:800, Genetex), NeuN (1:500, Temecula), Lbx1 (1:800, Novus), Cre (1:500, Millipore), GFAP (1:1000, Millipore), Alexa Fluor 488-conjugated AffiniPure goat anti-mouse or anti-rabbit IgG, Alexa Fluor 594-conjugated AffiniPure goat anti-mouse or anti-rabbit IgG (1:200, YEASEN), AffiniPure goat anti-chicken IgY (IgG) (H + L) (1:500, Jackson ImmunoResearch), and DAPI nuclear counterstain (1:1000, YEASEN).

Techniques: Expressing, Staining

Journal: Journal of Molecular Cell Biology

Article Title: Ablation of Zfhx4 results in early postnatal lethality by disrupting the respiratory center in mice

doi: 10.1093/jmcb/mjaa081

Figure Lengend Snippet: Zfhx4-positive neurons contribute to the development of the RTN. ( A ) Magnification of the ventral respiratory column from P0 WT mouse hindbrain, as indicated by the black rectangle in the inset model of the hindbrain (black ventral region is the RTN, while yellow circle indicates preBotc), showing the coexpression of Phox2b (red) and Zfhx4 (green) in VII and RTN neurons. DAPI was used to stain nuclei. ( B ‒ G ) Immunofluorescent staining and quantification for Phox2b, Math1, and Lbx1 in RTN neurons of P0 WT and Zfhx4 -null mice. RTN region was double-labelled by Phox2b (red) and Math1 or Lbx1 (green) in P0 WT (showing coexpression of Zfhx4 with Phox2b) and Zfhx4 -null mouse medulla. DAPI was used to stain nuclei. Further magnified images for the selected regions indicated by solid white rectangles are shown to the right. The expression of Phox2b, Math1, and Lbx1 was reduced in the RTN neurons of the P0 Zfhx4- null mice. Scale bar, 100 μm and 25 μm (further magnified images). Data in bar graph are expressed as mean ± SD. **** P < 0.0001 (Student’s t -test), n = 15.

Article Snippet: The antibodies and dilutions used in this study were as follows: Zfhx4 (1:2000), Phox2b (1:150, Santa Cruz), Math1 (1:800, Genetex), NeuN (1:500, Temecula), Lbx1 (1:800, Novus), Cre (1:500, Millipore), GFAP (1:1000, Millipore), Alexa Fluor 488-conjugated AffiniPure goat anti-mouse or anti-rabbit IgG, Alexa Fluor 594-conjugated AffiniPure goat anti-mouse or anti-rabbit IgG (1:200, YEASEN), AffiniPure goat anti-chicken IgY (IgG) (H + L) (1:500, Jackson ImmunoResearch), and DAPI nuclear counterstain (1:1000, YEASEN).

Techniques: Staining, Expressing